7. Project Summary/Abstract This S10 Shared Instrumentation Grant Proposal requests funds to acquire an Orbitrap Eclipse Tribrid mass spectrometer (MS). This new Orbitrap will replace our current LTQ Orbitrap XL MS and the TSQ Quantum Ultra MS, both more than 15 years old. This strategic investment will save significant taxpayers’ moneys by replacing two old instruments with just one to reduce the cost of operation. At the same time, the unique capabilities of the Orbitrap Eclipse would greatly enhance the research topics which can be analyzed to meet the needs of our research community, including identification of PTMs including phosphorylation, analysis of glycans, and top- down proteomics. The new Orbitrap will be housed in the Proteomics core facility, in the Center for Biotechnology and Interdisciplinary Studies (CBIS), at RPI. If our proposal is funded, this advanced technology will be available to 30 research groups in the New York Capital Region. There are 20-NIH funded research groups participating in this proposal, nineteen of these are major users of the proposed instrumentation. Overall, this NIH-funded group is very strong with R01, R44, R37, R21, RF1, R35, R03 and accounts for 85% of the AUT. Currently an Orbitrap Eclipse Tribrid mass spectrometer is not available in a radius of 150 miles from RPI and will become an excellent resource in the NY Capital region and beyond. Many of the research programs in this proposal are related to bioengineering, structural biology, molecular biophysics and biomanufacturing. These programs are related to many human diseases, including Alzheimer’s disease, glomerular disease, spinal cord injury, lung recellularization, Parkinson’s disease, circadian rhythm, microbiome in human gut, and immune mechanisms/diseases, cardiac chirality, cancer, cholesterol levels, all of these projects are NIH funded. The user base spans from users with little experience in mass spectrometry that send samples to the facility and fully rely on the expertise of the Proteomics Core director, to highly experienced mass spectroscopists. Dr. Zagorevski will play a key role in designing experiments, running experiments and analyzing the results, making critical scientific contributions to the mass spectrometry field. Under his oversight, the Proteomics core is one of the most successful cores in CBIS with more than 90 peer-reviewed publications in the last 5 years. His outstanding expertise, well-established administration and maintenance policies, commitment to support both major and minor users, and affordable rates for both internal and external users will ensure success of this instrument as a regional resource.

8. Project Narrative The Proteomics Core facility at Rensselaer Polytechnic Institute is requesting funds to replace two 15-year-old mass spectrometers (Orbitrap and Quantum) with one state-of-the-art Orbitrap Eclipse Tribrid MS, representing significant savings for taxpayers. The new instrument will enable critical experimental work that will significantly advance the biomedical research in NY Capital Region because this will be the first instrument with such capabilities in the area, meeting the expanded needs of over 30 key collaborators.