Summary Physical exercise activates a series of molecular events in multiple tissues to meet the demand for oxygen and substrate. Repetitive exercise causes tissue adaptation and provides broad health benefits. A fundamental understanding of the molecular mechanisms underlying the beneficial effects of exercise at a systems level is only beginning to be understood. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is an NIH Common Fund initiative created to accelerate the knowledge of exercise biology by systematically collecting detailed clinical, physiological, and molecular phenotypes in both animal models and in a racially and ethnically diverse cohort of men and women across the age span. MoTrPAC is a highly integrated and productive consortium organized into 5 units, each making unique contributions to populating the MoTrPAC DataHub. MoTrPAc Components include Preclinical Animal Study Sites (PASS), Clinical Centers (CCs), the Bioinformatics Core (BIC), the Consortium Coordinating Center (CCC) and Chemical Analysis Sites (CAS). This proposal describes one such CAS, the Georgia Comprehensive Lipidomics Unit for MoTrPAC. Our CAS is comprised of a team of analytical chemists, lipid biochemists, metabolomicists, systems biologists and bioinformaticians with expertise in advanced high-throughput chemical analytics and big data, specifically focusing on exercise-induced alterations of the lipidome. CAS studies are divided into 3 categories, Genomics/Epigenomics/Transcriptomics, Proteomics, and Metabolomics/Lipidomics to produce a draft molecular map of exercise. Through the Biospecimens Committee, a tissue analysis plan (TAP) for both preclinical rats and human tissues was created and approved by the MoTrPAC Steering Committee. CAS efforts have led to extensive molecular profiles of 6-month and 18-month- old male and female F344 rats following both exercise training and an acute exercise bout. These data are being analyzed by teams of MoTrPAC investigators and initial results have been submitted for publication. CAS has also produced an initial evaluation of blood, muscle and adipose biopsy samples collected from sedentary participants and those undergoing chronic endurance and resistance exercise training generated by the CC prior to pause of recruitment due to the SARS-CoV2 pandemic. To complete the MoTrPAC project, CAS investigators will continue to carry out studies with the following Specific Aims: Aim 1- to participate in consortium-wide efforts to analyze and publish expansive multiomics data obtained from multiple tissues collected by PASS investigators; Aim 2- to collaborate with MoTrPAC to implement temporal targeted and untargeted lipidomics profiling of blood, muscle and adipose tissue according to the TAP; Aim 3- To perform data analysis and transfer to the BIC through existing pipelines; Aim 4- to participate in MoTrPAC Committees, Subcommittees, and Working Groups, perform continuous quality assurance of data, develop analytical strategies, and prepare presentations and manuscripts to disseminate results.

Narrative Prolonged or intensive physical activity has a profound effect on the lipid profiles of a variety of organs and bodily fluids. Lipidomics technologies enable these lipid changes to be accurately mapped to yield a more complete scientific understanding of how exercise affect lipids as fuels, signaling messengers and structural molecules. We will continue to serve as the MoTrPAC Chemical Analysis Site devoted to both targeted and untargeted lipidomic studies using mass spectrometry technologies, and the associated data science and bioinformatic efforts to integrate the multiomic datasets being created by the Consortium.