The All of Us (AoU) Research Program aims to create one of most diverse health databases in history. To meet this ambitious goal and build upon the program’s progress, we are proposing to create the New York Coalition, a group of academic medical centers and partners with significant experience in engagement, recruitment, and retention of those underrepresented in biomedical research (UBR). As part of this unique collaboration, Mount Sinai Health System (Sinai), Weill Cornell Medicine (WCM), New York City Health+Hospitals (NYC H+H), the Institute for Family Health (IFH) and NYU Langone (NYU) will engage diverse communities, neighborhoods and clinical networks across New York to recruit 88,500 new participants and retain 35,400 participants for the program through proven, effective strategies. These include the facilitation of a diverse stakeholder engagement board, deployment of a research equity toolkit, pilot grants for community-based organizations who want to become recruitment sites for AoU, and the well-validated coordinator and community health worker training program used nationwide. We also have extensive experience recruiting participants for AoU as well as many other genomic and non-genomic studies across this partnership. Together, we will leverage years of long-standing collaboration to develop coordinated engagement, recruitment, and retention strategies to partner with underrepresented populations across NYC and significantly contribute to the success of AoU. This study is part of the NIH’s Helping to End Addiction Long-term (HEAL) initiative to speed scientific solutions to the national opioid public health crisis. The NIH HEAL Initiative bolsters research across NIH to improve treatment for opioid misuse and addiction.