The aim of this project is to develop an immunofluorescent assay system
capable of quantitating the secretion of cytokines by individual cells in
mixed cell populations by encapsulating individual cells in agarose gel
microdrops (GMD) and then measuring secretion of cytokine molecules after
appropriate stimulation. The combination of immunofluorescent staining of
secreted cytokines and cell surface labeling techniques will permit
simultaneous determination of the phenotype of cytokine-secreting cells by
using two or three-color flow cytometric analysis. the availability of
this assay system could become an important tool for basic research,
clinical diagnostic and industrial applications.
PROPOSED COMMERCIAL APPLICATION:
The majority of commercialized biotechnology products are cytokines. In
addition the cytokine research product market is in excess of $500 M. New
assay formats for quantitating cytokine secretion at the single cell level
would also impact clinical diagnostic research.
National Institute of Allergy and Infectious Diseases
CFDA Code
855
DUNS Number
858837388
UEI
Project Start Date
01-July-1998
Project End Date
31-December-1998
Budget Start Date
01-July-1998
Budget End Date
31-December-1998
Project Funding Information for 1998
Total Funding
$100,000
Direct Costs
$72,065
Indirect Costs
$27,935
Year
Funding IC
FY Total Cost by IC
1998
National Institute of Allergy and Infectious Diseases
$100,000
Year
Funding IC
FY Total Cost by IC
Sub Projects
No Sub Projects information available for 1R43AI043774-01
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No Outcomes available for 1R43AI043774-01
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