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Project Details

HEME PROTEIN STRUCTURE AND DYNAMICS

Project Number2R01GM033576-20

Contact PI/Project LeaderSPIRO, THOMAS G.

Awardee OrganizationPRINCETON UNIVERSITY

Description

Abstract Text

Studies of molecular structure and dynamics in heme proteins will be carried out using laser-excited resonance Raman spectroscopy. A theoretical and computational framework for extracting structural information from the spectra will be implemented using recently developed porphyrin force fields and molecular graphics. Protein-heme interactions in cytochrome c peroxidase will be investigated systematically with site- directed mutants. The dynamics of quaternary structure change in hemoglobin will be explored wit ultraviolet resonance Raman spectroscopy. Electron transfer and conformational coupling in cytochrome oxidase will be investigated with transient absorption and Raman techniques. These studies are expected to help uncover the molecular mechanisms whereby oxygen metabolism is linked to biological function through the motions of proteins.

Public Health Relevance Statement

Data not available.

NIH Spending Category

No NIH Spending Category available.

Project Terms

Raman spectrometrychemical modelsconformationcytochrome oxidaseelectron transporthemoglobinhemoproteinhemoprotein structurelaser spectrometryligandsmolecular rearrangementoxygenperoxidasesporphyrin structureprotein structure functionrespiratory oxygenationsite directed mutagenesisultraviolet spectrometry

Details

Contact PI/ Project Leader

Name

SPIRO, THOMAS G.

Title

Contact

Other PIs

Not Applicable

Program Official

Name

Contact

Email not available

Organization

Name

PRINCETON UNIVERSITY

City

PRINCETON

Country

UNITED STATES (US)

Department Type

Unavailable

Organization Type

SCHOOLS OF ARTS AND SCIENCES

State Code

Congressional District

Other Information

Opportunity Number

Study Section

Bioanalytical and Metallobiochemistry Study Section[BMT]

Fiscal Year

1989

Award Notice Date

17-March-1989

Administering Institutes or Centers

National Institute of General Medical Sciences

CFDA Code

DUNS Number

002484665

UEI

NJ1YPQXQG7U5

Project Start Date

01-April-1979

Project End Date

31-March-1994

Budget Start Date

01-April-1989

Budget End Date

31-March-1990

Project Funding Information for 1989

Total Funding

$343,733

Direct Costs

$269,685

Indirect Costs

$74,048

Year	Funding IC	FY Total Cost by IC
1989	National Institute of General Medical Sciences	$343,733

Sub Projects

No Sub Projects information available for 2R01GM033576-20

Publications

Publications are associated with projects, but cannot be identified with any particular year of the project or fiscal year of funding. This is due to the continuous and cumulative nature of knowledge generation across the life of a project and the sometimes long and variable publishing timeline. Similarly, for multi-component projects, publications are associated with the parent core project and not with individual sub-projects.

No Publications available for 2R01GM033576-20

Patents

No Patents information available for 2R01GM033576-20

Outcomes

The Project Outcomes shown here are displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed are those of the PI and do not necessarily reflect the views of the National Institutes of Health. NIH has not endorsed the content below.

No Outcomes available for 2R01GM033576-20

Clinical Studies

No Clinical Studies information available for 2R01GM033576-20

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