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Project Details

NEGATIVE REGULATORY ELEMENT OF HAMSTER MUC 1 PROMOTER

Project Number5R01HL063742-04

Contact PI/Project LeaderKIM, KWANG CHUL

Awardee OrganizationUNIVERSITY OF MARYLAND BALTIMORE

Description

Abstract Text

DESCRIPTION (Adapted from the Applicant's Abstract): MUC 1 is a transmembrane cell surface mucin best known for its upregulation in breast cancer. The PI observes that its expression correlates with goblet cell differentiation in hamster tracheal epithelial cells in vitro. His preliminary promoter analysis of the hamster Muc 1 gene cloned in his lab reveals the presence of a silencer (P-NRE) not previously described. He proposes conventional methods to (1) deduce the nucleotides comprising the silencer, (2) identify the protein binding to the silencer, (3) clone this protein and (4) perform studies to determine if and how the protein-DNA interaction mediates Muc 1 upregulation during goblet cell differentiation in vitro.

Public Health Relevance Statement

Data not available.

NIH Spending Category

No NIH Spending Category available.

Project Terms

DNA binding proteinaffinity chromatographybreast neoplasmscell differentiationcell growth regulationgel mobility shift assaygene expressiongene induction /repressiongenetic librarygenetic promoter elementgenetic regulationgenetic regulatory elementgoblet cellshamstersmessenger RNAmolecular cloningmucinsneoplastic cellnorthern blottingsnucleotidesprotein bindingrespiratory epitheliumtissue /cell culturetranscription factortransfection /expression vector

Details

Contact PI/ Project Leader

Name

KIM, KWANG CHUL

Title

Contact

Other PIs

Not Applicable

Program Official

Name

BANKS-SCHLEGEL, SUSAN P

Contact

Organization

Name

UNIVERSITY OF MARYLAND BALTIMORE

City

BALTIMORE

Country

UNITED STATES (US)

Department Type

PHARMACOLOGY

Organization Type

SCHOOLS OF PHARMACY

State Code

Congressional District

Other Information

Opportunity Number

Study Section

Lung Biology and Pathology Study Section[LBPA]

Fiscal Year

2003

Award Notice Date

Administering Institutes or Centers

National Heart Lung and Blood Institute

CFDA Code

838

DUNS Number

188435911

UEI

Z9CRZKD42ZT1

Project Start Date

01-February-2000

Project End Date

31-January-2006

Budget Start Date

01-February-2003

Budget End Date

31-January-2006

Project Funding Information for 2003

Total Funding

$185,959

Direct Costs

$125,225

Indirect Costs

$60,734

Year	Funding IC	FY Total Cost by IC
2003	National Heart Lung and Blood Institute	$185,959

Sub Projects

No Sub Projects information available for 5R01HL063742-04

Publications

Publications are associated with projects, but cannot be identified with any particular year of the project or fiscal year of funding. This is due to the continuous and cumulative nature of knowledge generation across the life of a project and the sometimes long and variable publishing timeline. Similarly, for multi-component projects, publications are associated with the parent core project and not with individual sub-projects.

No Publications available for 5R01HL063742-04

Patents

No Patents information available for 5R01HL063742-04

Outcomes

The Project Outcomes shown here are displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed are those of the PI and do not necessarily reflect the views of the National Institutes of Health. NIH has not endorsed the content below.

No Outcomes available for 5R01HL063742-04

Clinical Studies

No Clinical Studies information available for 5R01HL063742-04

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