This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Sorter Core provides expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. John Morgan is the full time stem cell/sorter technician. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. The FACS facility is also equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators work with the core and prepare their cell populations for sorting or analysis. The core personnel routinely separate Lin- Rho(lo)Ho(lo), Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core serves all projects in this COBRE carrying out both separations and analysis work. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.