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Project Details

The Knockout of the Mouse Muc5ac

Project Number1Z01ES102146-03 NIDB ANNUAL REPORT

Contact PI/Project LeaderLANGENBACH, ROBERT

Awardee OrganizationNATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

Description

Abstract Text

A targeting construct containing the neomycin and HSTK genes and 10 kb of DNA genomic homology was constructed to disrupt the Muc5ac gene by homologous recombination. The construct was electroporated into mouse ES cells and 2 PCR positive clones of 170 clones analyzed were obtained. PCR and genomic Southern analysis verified targeting. Chimeras have been generated and 21 germline agouties have been obtained. The heterozygous Muc5ac mice are now being breed to generate wild type and Muc5ac-/- mice generated for lung inflammation and gastric ulceration studies.

Public Health Relevance Statement

Data not available.

NIH Spending Category

BiotechnologyCancer**Digestive DiseasesGenetics

Project Terms

BreedingCellsChimera organismDNAGastric ulcerGenesGenomicsHumanInfectionInflammationKnockout MiceLungLung InflammationMolecularMucinsMusNeomycinPTGS2 genePathway interactionsPharmacologyPhysiologicalPolymerase Chain ReactionPredispositionReportingRoleStomachUlcerembryonic stem cellhomologous recombinationinsightmalignant stomach neoplasm

Details

Contact PI/ Project Leader

Name

LANGENBACH, ROBERT

Title

Contact

Other PIs

Not Applicable

Program Official

Name

Contact

Email not available

Organization

Name

NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

City

Country

UNITED STATES

Department Type

Unavailable

Organization Type

Unavailable

State Code

Congressional District

Other Information

Opportunity Number

Study Section

Fiscal Year

2008

Award Notice Date

Administering Institutes or Centers

National Institute of Environmental Health Sciences

CFDA Code

DUNS Number

UEI

Project Start Date

Project End Date

Budget Start Date

Budget End Date

Project Funding Information for 2008

Total Funding

$380,285

Direct Costs

Indirect Costs

Year	Funding IC	FY Total Cost by IC
2008	National Institute of Environmental Health Sciences	$380,285

NIH Categorical SpendingClick here for more information on NIH Categorical Spending

Funding IC	FY Total Cost by IC	NIH Spending Category
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES	$380,285	Biotechnology; Cancer; Digestive Diseases; Genetics

Sub Projects

No Sub Projects information available for 1Z01ES102146-03

Publications

Publications are associated with projects, but cannot be identified with any particular year of the project or fiscal year of funding. This is due to the continuous and cumulative nature of knowledge generation across the life of a project and the sometimes long and variable publishing timeline. Similarly, for multi-component projects, publications are associated with the parent core project and not with individual sub-projects.

No Publications available for 1Z01ES102146-03

Patents

No Patents information available for 1Z01ES102146-03

Outcomes

The Project Outcomes shown here are displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed are those of the PI and do not necessarily reflect the views of the National Institutes of Health. NIH has not endorsed the content below.

No Outcomes available for 1Z01ES102146-03

Clinical Studies

No Clinical Studies information available for 1Z01ES102146-03

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