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Project Details

Assay Dev/Adapt (California)

Parent Project Number5U54MH084512-03

Sub-Project ID0002

Contact PI/Project LeaderBROWN, STEVEN J

Awardee OrganizationSCRIPPS RESEARCH INSTITUTE, THE

Description

Abstract Text

ASSAY DEVELOPMENT/ADAPTATION CORE In La Jolla, 600 square ft of laboratory space is dedicated to the MLPCN effort. The lab is configured for maximal use of space with the tissue culture room adjacent to the automation bench. The tissue culture room has two 6-foot biosafety cabinets. In addition, the PI has 2500 sq. ft lab space immediately below the assay development facility. The lab is fully equipped for cell and molecular biology and is used in assay development efforts requiring these capabilities. The tissue culture lab has a Zeiss Axiovert fluorescent microscope equipped with filters for visual detection of green fluorescence (FITC, GFP) and CCF2/4 (b-lactamase substrate) loaded cells. The ICND building has a core microscopy facility available for secondary assays (e.g. GPCR internalization) with the following equipment. ¿ Brightfield and fluorescence microscopes ¿ Dissecting stereoscope with color camera ¿ Calcium imaging automated system with micromanipulator for cell picking Olympus confocal laser-scanning microscope with 4 laser lines ¿ DeltaVision deconvolution acquisition system ¿ Spinning-disk confocal microscope (2 laser lines) with incubator chamber for live-cell fluorescence microscopy ¿ Live-cell brightfield microscope with incubator ¿ Eppendorf microinjection system on inverted fluorescence microscope ¿ MetaMorph 6.5 acquisition and image analysis software

Public Health Relevance Statement

Data not available.

NIH Spending Category

Bioengineering

Project Terms

AdherenceApplications GrantsAutomationBiological AssayBusiness RulesCalciumCaliforniaCatalogingCatalogsCell CountCell LineCellsChemicalsChemistryCollaborationsColorComputer softwareCritical PathwaysCyclic AMPDataData FilesData QualityDatabasesDetectionDevelopmentDevelopment PlansDimethyl SulfoxideDoseEnsureEnzymesEquipmentEvaluationFloridaFluorescein-5-isothiocyanateFluorescenceFluorescence MicroscopyFollow-Up StudiesFreezingG Protein-Coupled Receptor GenesGeneric DrugsGoalsHandHourHousingImageImage AnalysisIncubatorsInformaticsLabelLaboratoriesLactamaseLasersLengthLifeMaintenanceMeasurementMeasuresMentorsMicroinjectionsMicroscopeMicroscopyMiniaturizationMolecular and Cellular BiologyMycoplasmaNucleic AcidsOutsourcingPeptidesPerformanceProceduresProcessProductionProteinsProtocols documentationProviderPubChemQuality ControlReadinessReagentRecordsReporterReportingResearch PersonnelResourcesRoboticsRunningSamplingScanningScheduleScienceScreening procedureSecureSourceSpecificityStreamSummary ReportsSurveysSynthesis ChemistrySystemTeleconferencesTemperatureTestingTimeTimeLineTitrationsUnited States National Institutes of HealthUpdateVendorVial deviceVisualWorkWorkplaceassay developmentbasecontinuous cell linecounterscreenenzyme substratefluorescence microscopefollow-upfootinhibitor/antagonistinterestmeetingsmembermicromanipulatoroutreachrelease of sequestered calcium ion into cytoplasmresponsescale upsquare foottissue culture

Details

Contact PI/ Project Leader

Name

BROWN, STEVEN J

Title

Contact

Other PIs

Not Applicable

Program Official

Name

Contact

Email not available

Organization

Name

SCRIPPS RESEARCH INSTITUTE, THE

City

LA JOLLA

Country

UNITED STATES (US)

Department Type

Unavailable

Organization Type

Other Domestic Non-Profits

State Code

Congressional District

Other Information

Opportunity Number

RFA-RM-08-005

Study Section

Special Emphasis Panel[ZRG1]

Fiscal Year

2010

Award Notice Date

Administering Institutes or Centers

National Institute of Mental Health

CFDA Code

DUNS Number

781613492

UEI

PHZJFZ32NKH4

Project Start Date

01-June-2010

Project End Date

31-May-2014

Budget Start Date

01-June-2010

Budget End Date

31-May-2011

Project Funding Information for 2010

Total Funding

$2,949,945

Direct Costs

$1,553,420

Indirect Costs

$1,396,525

Year	Funding IC	FY Total Cost by IC
2010	National Institute of Mental Health	$2,949,945

NIH Categorical SpendingClick here for more information on NIH Categorical Spending

Funding IC	FY Total Cost by IC	NIH Spending Category
RMAP	$2,949,945	Bioengineering

Sub Projects

No Sub Projects information available for 5U54MH084512-03 0002

Publications

Publications are associated with projects, but cannot be identified with any particular year of the project or fiscal year of funding. This is due to the continuous and cumulative nature of knowledge generation across the life of a project and the sometimes long and variable publishing timeline. Similarly, for multi-component projects, publications are associated with the parent core project and not with individual sub-projects.

No Publications available for 5U54MH084512-03 0002

Patents

No Patents information available for 5U54MH084512-03 0002

Outcomes

The Project Outcomes shown here are displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed are those of the PI and do not necessarily reflect the views of the National Institutes of Health. NIH has not endorsed the content below.

No Outcomes available for 5U54MH084512-03 0002

Clinical Studies

No Clinical Studies information available for 5U54MH084512-03 0002

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