In meeting the goals and objectives of FMIC, the center continues to: -Service and maintain state-of-the-art equipment for unlimited access by intramural scientists. -Provide investigator training in the use and analysis of fluorescence and optical-based techniques. -Develop and optimize experimental protocols in collaboration with intramural scientists. -Maintain and develop state-of-the-art analysis tools and protocols in collaboration with investigators -Monitor and incorporate emerging fluorescence and optical technologies that will support the mission of NIEHS into FMIC. During this fiscal year, FMIC has collaborated and supported 46 Principle Investigators from 8 branches/laboratories within the NIEHS DIR. Some publications from this FY resulting from work in the center is listed in the bibliography below: Bibliography Snyder RJ, Verhein KC, Vellers HL, Burkholder AB, Garantziotis S, Kleeberger SR. Multi-walled carbon nanotubes upregulate mitochondrial gene expression and trigger mitochondrial dysfunction in primary human bronchial epithelial cells. Nanotoxicology. 2019 Dec;13(10):1344-1361. Lin WC, Gowdy KM, Madenspacher JH, Zemans RL, Yamamoto K, Lyons-Cohen M, Nakano H, Janardhan K, Williams CJ, Cook DN, Mizgerd JP, Fessler MB. Epithelial membrane protein 2 governs transepithelial migration of neutrophils into the airspace. J Clin Invest. 2020 Jan 2;130(1):157-170. Sinha BK, Perera L, Cannon RE. Reversal of drug resistance by JS-K and nitric oxide in ABCB1- and ABCG2-expressing multi-drug resistant human tumor cells. Biomed Pharmacother. 2019 Dec. Shizu R, Yokobori K, Perera L, Pedersen L, Negishi M. Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci Rep. 2019 Nov 13. Janoshazi AK, Horton JK, Zhao ML, Prasad R, Scappini EL, Tucker CJ, Wilson SH. Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst). 2020 Jan. Tu D, Gao Y, Yang R, Guan T, Hong JS, Gao HM. The pentose phosphate pathway regulates chronic neuroinflammation and dopaminergic neurodegeneration. J Neuroinflammation. 2019 Dec 5. Sil P, Suwanpradid J, Muse G, Gruzdev A, Liu L, Corcoran DL, Willson CJ, Janardhan K, Grimm S, Myers P, Degraff LM, MacLeod AS, Martinez J. Noncanonical autophagy in dermal dendritic cells mediates immunosuppressive effects of UV exposure. J Allergy Clin Immunol. 2020 May. Gao Y, Tu D, Yang R, Chu CH, Hong JS, Gao HM. Through Reducing ROS Production, IL-10 Suppresses Caspase-1-Dependent IL-1 Maturation, thereby Preventing Chronic Neuroinflammation and Neurodegeneration. Int J Mol Sci. 2020 Jan. Jiang Z, Lai Y, Beaver JM, Tsegay PS, Zhao ML, Horton JK, Zamora M, Rein HL, Miralles F, Shaver M, Hutcheson JD, Agoulnik I, Wilson SH, Liu Y. Oxidative DNA Damage Modulates DNA Methylation Pattern in Human Breast Cancer 1 (BRCA1) Gene via the Crosstalk between DNA Polymerase and a de novo DNA Methyltransferase. Cells. 2020 Jan. Diaz-Jimenez D, Petrillo MG, Busada JT, Hermoso MA, Cidlowski JA. Glucocorticoids mobilize macrophages by transcriptionally up-regulating the exopeptidase DPP4. J Biol Chem. 2020 Mar 6;295(10):3213-3227. Tillage RP, Sciolino NR, Plummer NW, Lustberg D, Liles LC, Hsiang M, Powell JM, Smith KG, Jensen P, Weinshenker D. Elimination of galanin synthesis in noradrenergic neurons reduces galanin in select brain areas and promotes active coping behaviors. Brain Struct Funct. 2020 Mar;225(2):785-803. Zhao Z, Wang Y, Zhou R, Li Y, Gao Y, Tu D, Wilson B, Song S, Feng J, Hong JS, Yakel JL. A novel role of NLRP3-generated IL-1 in the acute-chronic transition of peripheral lipopolysaccharide-elicited neuroinflammation: implications for sepsis-associated neurodegeneration. J Neuroinflammation. 2020 Feb. Brown LY, Alexander GM, Cushman J, Dudek SM. Hippocampal CA2 Organizes CA1 Slow and Fast Oscillations during Novel Social and Object Interaction. eNeuro. 2020 Apr 14. Romeo C, Chen SH, Goulding E, Van Gorder L, Schwartz M, Walker M, Scott G, Scappini E, Ray M, Martin NP. AAV diffuses across zona pellucida for effortless gene delivery to fertilized eggs. Biochem Biophys Res Commun. 2020 May 21. Nadalutti CA, Stefanick DF, Zhao ML, Horton JK, Prasad R, Brooks AM, Griffith JD, Wilson SH. Mitochondrial dysfunction and DNA damage accompany enhanced levels of formaldehyde in cultured primary human fibroblasts. Scientific Reports. 2020 Mar 27. Cannon RE, Richards AC, Trexler AW, Juberg CT, Sinha B, Knudsen GA, Birnbaum LS. Effect of GenX on P-Glycoprotein, Breast Cancer Resistance Protein, and Multidrug Resistance-Associated Protein 2 at the Blood-Brain Barrier. Environ Health Perspect. 2020 Mar. Plummer NW, Chandler DJ, Powell JM, Scappini EL, Waterhouse BD, Jensen P. An Intersectional Viral-Genetic Method for Fluorescent Tracing of Axon Collaterals Reveals Details of Noradrenergic Locus Coeruleus Structure. eNeuro. 2020 May 15. Chen SH, Papaneri A, Walker M, Scappini E, Keys RD, Martin NP. A Simple, Two-Step, Small-Scale Purification of Recombinant Adeno-Associated Viruses. J Virol Methods. 2020 Jul. Gu Z, Smith KG, Alexander GM, Guerreiro I, Dudek SM, Gutkin B, Jensen P, Yakel JL. Hippocampal Interneuronal 7 nAChRs Modulate Theta Oscillations in Freely Moving Mice. Cell Rep. 2020 Jun 9. Howard MJ, Horton JK, Zhao ML, Wilson SH. Lysines in the lyase active site of DNA polymerase destabilize nonspecific DNA binding, facilitating searching and DNA gap recognition. J Biol Chem. 2020 Aug 21. Scoville DW, Gruzdev A, Jetten AM. Identification of a novel lncRNA (G3R1) regulated by GLIS3 in pancreatic -cells. J Mol Endocrinol. 2020 Oct. Sahu S, Wang Z, Jiao X, Gu C, Jork N, Wittwer C, Li X, Hostachy S, Fiedler D, Wang H, Jessen HJ, Kiledjian M, Shears SB. InsP(7) is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics. Proc Natl Acad Sci U S A. 2020 Aug 11. Shindo S, Chen SH, Gotoh S, Yokobori K, Hu H, Ray M, Moore R, Nagata K, Martinez J, Hong JS, Negishi M. Estrogen receptor phosphorylated at Ser216 confers inflammatory function to mouse microglia. Cell Commun Signal. 2020 Jul 29. Mazzone CM, Liang-Guallpa J, Li C, Wolcott NS, Boone MH, Southern M, Kobzar NP, Salgado IA, Reddy DM, Sun F, Zhang Y, Li Y, Cui G, Krashes MJ. High-fat food biases hypothalamic and mesolimbic expression of consummatory drives. Nat Neurosci. 2020 Aug 3.